Tested Applications
| Positive WB detected in | pig lung tissue, rat lung tissue |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat lung tissue, mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66833-1-Ig targets AGER/RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9657 Product name: Recombinant human AGER protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-340 aa of BC020669 Sequence: ITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGT Predict reactive species |
| Full Name | advanced glycosylation end product-specific receptor |
| Calculated Molecular Weight | 404 aa, 43 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC020669 |
| Gene Symbol | AGER |
| Gene ID (NCBI) | 177 |
| RRID | AB_2882176 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15109 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| IHC protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| WB protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AGER/RAGE Monoclonal antibody (catalog #66833-1-Ig) has 14 total citations. They appear in Journal of Ethnopharmacology (4), International Immunopharmacology, Cell Oncology, Biomolecules, Cellular Signalling, Genes & Immunity, Molecular Immunology, Biochemistry and Biophysics Reports, IBRO Neuroscience Reports, Chemistry & Biodiversity, and Applied Biochemistry and Biotechnology. Research fields include cerebral ischemia-reperfusion injury, rheumatoid arthritis, NAFLD, hypertensive nephropathy, gastric ulcer, breast cancer, Alzheimer's disease, podocyte injury, pulmonary fibrosis, nasal epithelial inflammation, nerve regeneration, polycystic ovary syndrome, and diabetic nephropathy.
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Zhongfeng Decoction attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy via regulating the AGE-RAGE signaling pathway | ||
J Ethnopharmacol Qinggan Jiangzhi Cha ameliorates NAFLD by modulating the AGE-RAGE/PRKCA/MAPK3/AP-1 signaling axis. | ||
J Ethnopharmacol Huanglian Jiedu Decoction ameliorates Hypertensive Nephropathy by inhibiting the AGEs-RAGE/MAPK/AP-1 axis: An integrated transcriptomics and network pharmacology study. | ||
J Ethnopharmacol Siwei Zangmu Xiang alleviates rheumatoid arthritis via regulating RAGE signaling pathway: Integration of UPLC-Q-TOF-MS, network pharmacology, and in vivo/in vitro studies. | ||
Cell Oncol (Dordr) P2RY6(+) tumor-associated macrophages as a therapeutic target: suppressing tumor growth and enhancing chemosensitivity in triple-negative breast cancer. | ||
Biomolecules Evaluation of the Effects of Thymoquinone on RAGE/NOX4 Expressions and Brain Tissue Morphometry in Experimental Alzheimer's Disease Induced by Amyloid Beta 1-42 Peptide |
