AGER Monoclonal antibody

AGER Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat and More (2)

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

3D4G11

Cat no : 66833-1-Ig

Synonyms

AGER, RAGE



Tested Applications

Positive WB detected inpig lung tissue, rat lung tissue
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66833-1-Ig targets AGER in WB, IHC applications and shows reactivity with Human, Pig, Rat samples.

Tested Reactivity Human, Pig, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AGER fusion protein Ag9657
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight 404 aa, 43 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC020669
Gene symbol AGER
Gene ID (NCBI) 177
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

Protocols

Product Specific Protocols
WB protocol for AGER antibody 66833-1-IgDownload protocol
IHC protocol for AGER antibody 66833-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Immunol

S100A11 regulates nasal epithelial cell remodeling and inflammation in CRSwNPs via the RAGE-mediated AMPK-STAT3 pathway.

Authors - Chengcheng Liu
mouseWB

Cell Signal

Dapagliflozin alleviates advanced glycation end product induced podocyte injury through AMPK/mTOR mediated autophagy pathway

Authors - Lei Yang