AGFG2 Polyclonal antibody

AGFG2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11919-1-AP

Synonyms

AGFG2, ArfGAP with FG repeats 2, HRBL, RAB R, RABR

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Validation Data Gallery

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  WB analysis of Jurkat using 11919-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11919-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11919-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 11919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11919-1-AP (AGFG2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11919-1-AP

  Immunofluorescent analysis of HepG2 cells, using AGFG2 antibody 11919-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11919-1-AP targets AGFG2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGFG2 fusion protein Ag2546
• Full Name: ArfGAP with FG repeats 2
• Calculated Molecular Weight: 481 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC017329
• Gene Symbol: AGFG2
• Gene ID (NCBI): 3268
• RRID: AB_2224646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.