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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AGO2 Monoclonal antibody

AGO2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G2H12

Cat no : CL488-67934

Synonyms

AGO2, Argonaute2, eIF 2C 2, eIF2C 2, EIF2C2, hAgo2, PAZ Piwi domain protein, PPD, Protein argonaute 2, Protein slicer, Q10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated AGO2 Monoclonal anti (CL488-67934)

    IF Staining of HeLa using CL488-67934

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 AGO2 antibody (CL488-67934, Clone: 1G2H12 ) at dilution of 1:1000.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated AGO2 Monoclonal anti (CL488-67934)

    FC experiment of HeLa using CL488-67934

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AGO2 (CL488-67934, Clone:1G2H12) (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67934 targets AGO2 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AGO2 fusion protein Ag27772
Full Name eukaryotic translation initiation factor 2C, 2
Calculated Molecular Weight 97 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC007633
Gene Symbol EIF2C2
Gene ID (NCBI) 27161
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AGO2 antibody CL488-67934Download protocol
FC protocol for CL Plus 488 AGO2 antibody CL488-67934Download protocol
Standard Protocols
Click here to view our Standard Protocols