AGTR1 Monoclonal antibody

AGTR1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C5G11

Cat no : 66415-1-Ig

Synonyms

AG2S, AGTR1, AGTR1A, AGTR1B, Angiotensin II type 1 receptor, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, AT2R1A, AT2R1B, HAT1R, Type 1 angiotensin II receptor



Tested Applications

Positive WB detected inpig heart tissue, pig skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66415-1-Ig targets AGTR1 in WB, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AGTR1 fusion protein Ag14461
Full Name angiotensin II receptor, type 1
Calculated molecular weight 359 aa, 41 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC022447
Gene symbol AGTR1
Gene ID (NCBI) 185
RRIDAB_2881787
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named as AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Protocols

Product Specific Protocols
WB protocol for AGTR1 antibody 66415-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Adv Res

Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system.

Authors - Han Su

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anca (Verified Customer) (10-30-2019)

We could detect AT1R from Plazenta, but only by using Chemiluminiscence. Unfortunately, the pozitive control that we used didn't worked.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Plazenta