CoraLite® Plus 488-conjugated AGXT Recombinant monoclonal antibody

• 

  IF Staining of HeLa using CL488-83385-6

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 AGXT antibody (CL488-83385-6, Clone: 240305E7 ) at dilution of 1:200.

AGXT Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-83385-6

Clone No.240305E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83385-6 targets AGXT in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18062

  Product name: Recombinant human AGXT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-392 aa of BC132819

  Sequence: MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL

• Full Name: alanine-glyoxylate aminotransferase
• Calculated Molecular Weight: 392 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC132819
• Gene Symbol: AGXT
• Gene ID (NCBI): 189
• RRID: AB_3673245
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21549
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.