Filter: AllWBIHCIF

WB analysis using 66019-1-Ig (same clone as 66019-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 66019-1-Ig (same clone as 66019-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 66019-1-Ig (same clone as 66019-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 66019-1-Ig (same clone as 66019-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

AHCY Monoclonal antibody, PBS Only

AHCY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66019-1-PBS

Clone No.1F9B10

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66019-1-PBS targets AHCY in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag18036 Product name: Recombinant human AHCY protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 116-432 aa of BC011606 Sequence: QTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY Predict reactive species
Full Name	S-adenosylhomocysteine hydrolase
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC011606
Gene Symbol	AHCY
Gene ID (NCBI)	191
RRID	AB_11044194
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P23526
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).