AHCY Monoclonal antibody, PBS Only

AHCY Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9B10

Cat no : 66019-1-PBS

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

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Validation Data Gallery

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  WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66019-1-Ig (same clone as 66019-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66019-1-Ig (same clone as 66019-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66019-1-PBS targets AHCY in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: AHCY fusion protein Ag18036
• Full Name: S-adenosylhomocysteine hydrolase
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC011606
• Gene Symbol: AHCY
• Gene ID (NCBI): 191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).