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  • KD/KO Validated

AHI1 Polyclonal antibody

AHI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 22045-1-AP

Synonyms

AHI 1, AHI1, JBTS3, Jouberin, ORF1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cell

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22045-1-AP targets AHI1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHI1 fusion protein Ag16885
Full Name Abelson helper integration site 1
Calculated Molecular Weight 1196 aa, 137 kDa
Observed Molecular Weight 137-150 kDa
GenBank Accession NumberBC094800
Gene Symbol AHI1
Gene ID (NCBI) 54806
RRIDAB_11182927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AHI1, encoding the Jouberin protein, is an alternatively spliced signaling molecule that contains seven Trp-Asp (WD) repeats, an SH3 domain, and numerous SH3-binding sites. AHI1 is expressed strongly in embryonic hindbrain and forebrain. AHI1 is involved in vesicle trafficking and is required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. AHI1, as a positive modulator of classical Wnt signaling, may play a key role in ciliary signaling during cerebellar embryonic development (PMID: 15467982, PMID: 21623382).

Protocols

Product Specific Protocols
WB protocol for AHI1 antibody 22045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

Authors - Kari-Anne M Frikstad
humanIF

Front Cell Dev Biol

Superresolution Microscopy Reveals Distinct Phosphoinositide Subdomains Within the Cilia Transition Zone.

Authors - Sarah E Conduit
mouseIF,WB

Mol Biol Cell

Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function.

Authors - Yin Loon Lee
  • KD Validated
humanIF

Sci Rep

Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.

Authors - Elisa Molinari
mouseIF

Front Cell Dev Biol

EVC-EVC2 complex stability and ciliary targeting are regulated by modification with ubiquitin and SUMO

Authors - Pablo Barbeito