CoraLite® Plus 488-conjugated AHR Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-83369-6

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 AHR antibody (CL488-83369-6, Clone: 240325G9 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-83369-6

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated AHR Recombinant monoclonal antibody (CL488-83369-6, Clone: 240325G9) (red) or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

AHR Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL488-83369-6

Clone No.240325G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

240325G9, Ah receptor, bHLHe76, Class E basic helix-loop-helix protein 76

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83369-6 targets AHR in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12193

  Product name: Recombinant human AHR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-387 aa of BC070080

  Sequence: MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQGKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGSGYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLT

• Full Name: aryl hydrocarbon receptor
• Calculated Molecular Weight: 848 aa, 96 kDa
• GenBank Accession Number: BC070080
• Gene Symbol: AHR
• Gene ID (NCBI): 196
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35869
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH) 17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)