AIF Monoclonal antibody

AIF Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

8C12B2

Cat no : 67791-1-Ig

Synonyms

AIF, AIFM1, PDCD8



Tested Applications

Positive WB detected inLNCaP cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells, MDCK cells, Pig brain cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, human kidney tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67791-1-Ig targets AIF in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AIF fusion protein Ag12400
Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Calculated molecular weight 609 aa, 66 kDa
Observed molecular weight 67 kDa, 57 kDa
GenBank accession numberBC111065
Gene symbol AIFM1
Gene ID (NCBI) 9131
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).

Protocols

Product Specific Protocols
WB protocol for AIF antibody 67791-1-IgDownload protocol
IHC protocol for AIF antibody 67791-1-IgDownload protocol
IF protocol for AIF antibody 67791-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols