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- KD/KO Validated
AIF Monoclonal antibody, PBS Only
AIF Monoclonal Antibody for WB, IF, IHC, Indirect ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig, Canine
Applications
WB, IF, IHC, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
8C12B2
Cat no : 67791-1-PBS
Synonyms
Validation Data Gallery
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67791-1-PBS targets AIF in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Canine samples.
Tested Reactivity | Human, Mouse, Rat, Pig, Canine |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | AIF fusion protein Ag12400 |
Full Name | apoptosis-inducing factor, mitochondrion-associated, 1 |
Calculated Molecular Weight | 609 aa, 66 kDa |
Observed Molecular Weight | 67 kDa, 57 kDa |
GenBank Accession Number | BC111065 |
Gene Symbol | AIFM1 |
Gene ID (NCBI) | 9131 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).