AIF Recombinant antibody, PBS Only (Detector)

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  WB analysis using 86156-1-RR (same clone as 86156-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86156-1-RR (AIF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86156-1-PBS in a different storage buffer formulation.

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  Sample test of MP02287-1

  The mean AIF concentration was determined to be 20.2 ng/mL in Hela cell extract based on a 0.9 mg/mL extract load.

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  IF Staining of HeLa using 86156-1-RR (same clone as 86156-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIF antibody (86156-1-RR, Clone: 250772G11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86156-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02287-1

  Cytometric bead array standard curve of MP02287-1, AIF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86156-2-PBS. Detection antibody: 86156-1-PBS. Standard: Ag13268. Range: 0.781-100 ng/mL.

AIF Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86156-1-PBS

Clone No.250772G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8

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Product Information

86156-1-PBS targets AIF as part of a matched antibody pair:

MP02287-1: 86156-2-PBS capture and 86156-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AIF fusion protein Ag13268
• Full Name: apoptosis-inducing factor, mitochondrion-associated, 1
• Calculated Molecular Weight: 609 aa, 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC111065
• Gene Symbol: AIF
• Gene ID (NCBI): 9131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95831
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).