AIRE Polyclonal antibody

AIRE Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22517-1-AP

Synonyms

AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1



Tested Applications

Positive WB detected inL02 cells, human plasma tissue, human spleen tissue, mouse thymus tissue, U-937 cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22517-1-AP is AIRE Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIRE fusion protein Ag18249
Full Name autoimmune regulator
Calculated molecular weight 545 aa, 58 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC137270
Gene symbol AIRE
Gene ID (NCBI) 326
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The function of the protein is not well defined, however it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Protocols

Product Specific Protocols
WB protocol for AIRE antibody 22517-1-APDownload protocol
IHC protocol for AIRE antibody 22517-1-APDownload protocol
IF protocol for AIRE antibody 22517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Immunol

Chd4 choreographs self-antigen expression for central immune tolerance.

Authors - Yoshihiko Tomofuji