AK1 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85979-3-RR (same clone as 85979-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85979-3-RR (AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85979-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 85979-3-RR (same clone as 85979-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85979-3-RR (AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85979-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02232-2

  Sandwich ELISA standard curve of MP02232-2, Human AK1 Recombinant Matched Antibody Pair - PBS only. 85979-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6890. 85979-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02232-2

  The mean AK1 concentration was determined to be 35.20 ng/mL in HeLa cell extract based on a 0.9 mg/mL extract load.

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  Sample test of MP02232-2

  Human milk of six individual healthy human donors was measured. The human AK1 concentration of detected samples was determined to be 2.83 ng/mL with a range of 0.76 - 8.63 ng/mL.

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  Affinity and Kinetic Characterization of 85979-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85979-3-RR against Human AK1 were performed. The affinity constant is below 1 pM.

AK1 Uni-rAb^TM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85979-3-PBS

Clone No.250557D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

adenylate kinase 1, Adenylate kinase isoenzyme 1, Adenylate monophosphate kinase, AK 1, ATP:AMP phosphotransferase

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Product Information

85979-3-PBS targets AK1 as part of a matched antibody pair:

MP02232-2: 85979-5-PBS capture and 85979-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AK1 fusion protein Ag6890
• Full Name: adenylate kinase 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC001116
• Gene Symbol: AK1
• Gene ID (NCBI): 203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00568
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AK1(Adenylate kinase isoenzyme 1), also known as ATP-AMP transphosphorylase 1. It is located in cytoplasm. This protein is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP 1. Exhibits nucleoside diphosphate kinase activity, catalyzing the production of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donor 2. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP. The molecular weight of AK1 is 22 kDa.