|Positive WB detected in||HeLa cells, HEK-293 cells, human heart tissue, human liver tissue, Jurkat cells, Raji cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human pancreas cancer tissue, human brain tissue, human testis tissue, human lung tissue, human kidney tissue, human skin tissue, human heart tissue, human ovary tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11014-1-AP targets AK2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AK2 fusion protein Ag1464|
|Full Name||adenylate kinase 2|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC009405|
|Gene ID (NCBI)||204|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for AK2 antibody 11014-1-AP||Download protocol|
|IHC protocol for AK2 antibody 11014-1-AP||Download protocol|
|IF protocol for AK2 antibody 11014-1-AP||Download protocol|
|IP protocol for AK2 antibody 11014-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Rhythmic nucleotide synthesis in the liver: temporal segregation of metabolites.
Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.
Reticular dysgenesis (aleukocytosis) is caused by mutations in the gene encoding mitochondrial adenylate kinase 2.