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AK3 Polyclonal antibody, PBS Only (12562-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 12562-1-AP (same clone as 12562-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.
WB analysis using 12562-1-AP (same clone as 12562-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.
IP experiment of mouse kidney using 12562-1-AP (same clone as 12562-1-PBS)
IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12562-1-AP (same clone as 12562-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 12562-1-AP (same clone as 12562-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 12562-1-AP (same clone as 12562-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12562-1-AP (same clone as 12562-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AK3 antibody (12562-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

AK3 Polyclonal antibody, PBS Only

Name: AK3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12562-1-PBS

AK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12562-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Adenylate kinase 3 alpha-like 1, AK 3, AK3L1, AK6, AKL3L

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12562-1-PBS targets AK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3146 Product name: Recombinant human AK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC013771 Sequence: MGASARLLRAVIMGAPGSGKGTVSSRITTHFELKHLSSGDLLRDNMLRGTEIGVLAKAFIDQGKLIPDDVMTRLALHELKNLTQYSWLLDGFPRTLPQAEALDRAYQIDTVINLNVPFEVIKQRLTARWIHPASGRVYNIEFNPPKTVGIDDLTGEPLIQREDDKPETVIKRLKAYEDQTKPVLEYYQKKGVLETFSGTETNKIWPYVYAFLQTKVPQRSQKASVTP Predict reactive species
Full Name	adenylate kinase 3
Calculated Molecular Weight	227 aa, 26 kDa
Observed Molecular Weight	26 kDa
GenBank Accession Number	BC013771
Gene Symbol	AK3
Gene ID (NCBI)	50808
RRID	AB_2305373
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UIJ7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.