AK3L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AK3L1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13206-1-AP

Synonyms

Adenylate kinase 3 like, adenylate kinase 3 like 1, AK3, AK3L1, AK4, ATP AMP transphosphorylase

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Validation Data Gallery

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  WB analysis of MCF-7 using 13206-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13206-1-AP (AK3L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13206-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13206-1-AP (AK3L1 antibody) at dilution of 1:1800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 13206-1-AP

  IP result of anti-AK3L1 (IP:13206-1-AP, 4ug; Detection:13206-1-AP 1:300) with MCF-7 cells lysate 3200 ug.

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  IHC staining of human breast cancer using 13206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13206-1-AP (AK3L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13206-1-AP (AK3L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13206-1-AP (AK3L1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, human liver tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3600
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13206-1-AP targets AK3L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AK3L1 fusion protein Ag3966
• Full Name: adenylate kinase 3-like 1
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC040224
• Gene Symbol: AK3L1
• Gene ID (NCBI): 205
• RRID: AB_2225456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the adenine and guanine nucleotide pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.

Publications

Species	Application	Title
human	WB	Cancer Cell Int; The miR-199a-3p regulates the radioresistance of esophageal cancer cells via targeting the AK4 gene.; Authors - Chunbao Zang ; KD Validated
rat	WB,IHC	Exp Brain Res; MicroRNA-124 conducts neuroprotective effect via inhibiting AK4/ATF3 after subarachnoid hemorrhage; Authors - Wei Jiang