AKAP1 Polyclonal antibody

AKAP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15618-1-AP

Synonyms

AKAP, AKAP 149, AKAP1, AKAP121, AKAP149, AKAP84, D AKAP 1, D AKAP1, PRKA1, S AKAP84, SAKAP84



Tested Applications

Positive WB detected inHeLa cells, human liver tissue, MCF-7 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue, human colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15618-1-AP targets AKAP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP1 fusion protein Ag8037
Full Name A kinase (PRKA) anchor protein 1
Calculated molecular weight 97 kDa
Observed molecular weight 149 kDa
GenBank accession numberBC000729
Gene symbol AKAP1
Gene ID (NCBI) 8165
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Protocols

Product Specific Protocols
WB protocol for AKAP1 antibody 15618-1-APDownload protocol
IHC protocol for AKAP1 antibody 15618-1-APDownload protocol
IF protocol for AKAP1 antibody 15618-1-APDownload protocol
IP protocol for AKAP1 antibody 15618-1-APDownload protocol
FC protocol for AKAP1 antibody 15618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,IF

Mol Endocrinol

Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis.

Authors - Grozdanov Petar N PN
humanWB

EMBO J

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Authors - Wenxian Wu