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AKD2 Polyclonal antibody, PBS Only (27554-1-PBS)

Filter: AllWBIHC

WB analysis using 27554-1-AP (same clone as 27554-1-PBS)
Various lysates lysate were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.
WB analysis of SMMC-7721 using 27554-1-AP (same clone as 27554-1-PBS)
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.
WB analysis of HuH-7 using 27554-1-AP (same clone as 27554-1-PBS)
HuH-7 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 27554-1-AP (same clone as 27554-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 27554-1-AP (same clone as 27554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 27554-1-AP (same clone as 27554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 27554-1-AP (same clone as 27554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 27554-1-AP (same clone as 27554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

AKD2 Polyclonal antibody, PBS Only

Name: AKD2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 27554-1-PBS

AKD2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27554-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

AK9, Adenylate kinase domain-containing protein 1, Adenylate kinase domain-containing protein 2, AK 9, AKD1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

27554-1-PBS targets AKD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25805 Product name: Recombinant human AKD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-421 aa of BC022031 Sequence: MTSQEKTEEYPFADIFDEDETERNFLLSKPVCFVVFGKPGVGKTTLARYITQAWKCIRVEALPILEEQIAAETESGVMLQSMLISGQSIPDELVIKLMLEKLNSPEVCHFGYIITEIPSLSQDAMTTLQQIELIKNLNLKPDVIINIKCPDYDLCQRISGQRQHNNTGYIYSRDQWDPEVIENHRKKKKEAQKDGKGEEEEEEEEQEEEEAFIAEMQMVAEILHHLVQRPEDYLENVENIVKLYKETILQTLEEVMAEHNPQYLIELNGNKPAEELFMIVMDRLKYLNLKRAAILTKLQGAEEEINDTMENDELFRTLASYKLIAPRYRWQRSKWGRTCPVNLKDGNIYSGLPDYSVSFLGKIYCLSSEEALKPFLLNPRPYLLPPMPGPPCKVFILGPQYSGKTTLCNMLAENYKGKVTN Predict reactive species
Full Name	adenylate kinase domain containing 2
Calculated Molecular Weight	221 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC022031
Gene Symbol	AKD2
Gene ID (NCBI)	221264
RRID	AB_2880904
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5TCS8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).