Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody, PBS Only

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  IF Staining of hTERT-RPE1 using 65611-1-MR (same clone as 65611-1-PBS)

  Immunofluorescent analysis of un-fixed hTERT-RPE1 cells using ALCAM antibody (65611-1-MR, Clone: 3A6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 65611-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65611-1-MR (same clone as 65611-1-PBS)

  1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Untreated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (black). Cells were not fixed. This data was developed using the same antibody clone with 65611-1-PBS in a different storage buffer formulation.

ALCAM Uni-rAb^® Recombinant Antibody for IF/ICC, FC

Cat No. 65611-1-PBS

Clone No.3A6

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC

Synonyms

MEMD, CD166, 3A6

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Product Information

65611-1-PBS targets ALCAM in IF/ICC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Cultured human thymic epithelial cells

• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 105 kDa
• GenBank Accession Number: BC057809
• Gene Symbol: ALCAM
• Gene ID (NCBI): 214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13740
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).