ALDH1A1 Monoclonal antibody

• Featured Product
• KD/KO Validated

ALDH1A1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

1A10A2

Cat no : 60171-1-Ig

Synonyms

ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC, 3-deoxyglucosone dehydrogenase

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Validation Data Gallery

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  WB analysis using 60171-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of K-562 using 60171-1-Ig

  WB result of ALDH1A1 antibody (60171-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells.

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  WB analysis of MCF-7 using 60171-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 60171-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 60171-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 60171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human brain using 60171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HeLa using 60171-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDH1A1 antibody (60171-1-Ig, Clone: 1A10A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 60171-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (60171-1-Ig, Clone:1A10A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, pig liver tissue, rat liver tissue, K-562 cells, HepG2 cells, HuH-7 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

60171-1-Ig targets ALDH1A1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDH1A1 fusion protein Ag8551
• Full Name: aldehyde dehydrogenase 1 family, member A1
• Calculated Molecular Weight: 501 aa, 55 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001505
• Gene Symbol: ALDH1A1
• Gene ID (NCBI): 216
• RRID: AB_10693634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).

Publications

Species	Application	Title
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
human	IF	Cell Rep Med; Targeting EMSY-mediated methionine metabolism is a potential therapeutic strategy for triple-negative breast cancer; Authors - Cui-Cui Liu
mouse	IF	Elife; Perirenal adipose tissue contains a subpopulation of cold-inducible adipocytes derived from brown-to-white conversion; Authors - Houyu Zhang
human	WB,CoIP	iScience; Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression; Authors - Zhilei Zhang ; KD Validated
human	WB	Front Pharmacol; Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells.; Authors - Ming Xin
human	WB	Oral Dis; microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1.; Authors - Kaixin Wangzhou

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C