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ALDH1A1 Monoclonal antibody

ALDH1A1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, pig







Cat no : 60171-1-Ig


ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

Tested Applications

Positive WB detected inMCF7 cell, MCF-7 cells, A549 cells, pig liver tissue, rat liver tissue, K-562 cells, HepG2 cells, HuH-7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A431 cells, mouse colon tissue
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

60171-1-Ig targets ALDH1A1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALDH1A1 fusion protein Ag8551
Full Name aldehyde dehydrogenase 1 family, member A1
Calculated molecular weight 501 aa, 55 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001505
Gene symbol ALDH1A1
Gene ID (NCBI) 216
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).


Product Specific Protocols
WB protocol for ALDH1A1 antibody 60171-1-IgDownload protocol
IHC protocol for ALDH1A1 antibody 60171-1-IgDownload protocol
IF protocol for ALDH1A1 antibody 60171-1-IgDownload protocol
FC protocol for ALDH1A1 antibody 60171-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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