ALDH1A1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ALDH1A1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10A2

Cat no : 60171-1-PBS

Synonyms

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

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Validation Data Gallery

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  WB analysis of K-562 using 60171-1-Ig (same clone as 60171-1-PBS)

  WB result of ALDH1A1 antibody (60171-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  WB analysis using 60171-1-Ig (same clone as 60171-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60171-1-Ig (same clone as 60171-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 60171-1-Ig (same clone as 60171-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 60171-1-Ig (same clone as 60171-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDH1A1 antibody (60171-1-Ig, Clone: 1A10A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 60171-1-Ig (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 60171-1-Ig (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunofluorescent analysis of A431 cells using 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 60171-1-Ig (same clone as 60171-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (60171-1-Ig, Clone:1A10A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60171-1-PBS targets ALDH1A1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDH1A1 fusion protein Ag8551
• Full Name: aldehyde dehydrogenase 1 family, member A1
• Calculated Molecular Weight: 501 aa, 55 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001505
• Gene Symbol: ALDH1A1
• Gene ID (NCBI): 216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).