ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22220-1-AP

Synonyms

EC:1.2.1.3, ALDHX, ALDH5, Aldehyde dehydrogenase X, mitochondrial, Aldehyde dehydrogenase 5



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22220-1-AP targets ALDH1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1B1 fusion protein Ag17557
Full Name aldehyde dehydrogenase 1 family, member B1
Calculated Molecular Weight 517 aa, 57 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC001619
Gene Symbol ALDH1B1
Gene ID (NCBI) 219
RRIDAB_2879035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

Protocols

Product Specific Protocols
WB protocol for ALDH1B1 antibody 22220-1-APDownload protocol
IHC protocol for ALDH1B1 antibody 22220-1-APDownload protocol
IF protocol for ALDH1B1 antibody 22220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oncotarget

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Authors - Hao-Long Zeng
humanIHC

Transl Cancer Res

ALDH1B1 predicts poor survival for locally advanced nasopharyngeal carcinoma patients.

Authors - Tao Zhu