ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22220-1-AP

Synonyms

EC:1.2.1.3, ALDHX, ALDH5, Aldehyde dehydrogenase X, mitochondrial, Aldehyde dehydrogenase 5

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Validation Data Gallery

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  WB analysis of HeLa using 22220-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22220-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22220-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22220-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 22220-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

• 

  IF Staining of MCF-7 using 22220-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ALDH1B1 antibody 22220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, rat brain tissue
• Positive IHC detected in: human ovary tumor tissue, human colon cancer tissue, human liver tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

22220-1-AP targets ALDH1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH1B1 fusion protein Ag17557
• Full Name: aldehyde dehydrogenase 1 family, member B1
• Calculated Molecular Weight: 517 aa, 57 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC001619
• Gene Symbol: ALDH1B1
• Gene ID (NCBI): 219
• RRID: AB_2879035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

Publications

Species	Application	Title
rat	WB	Oncotarget; Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.; Authors - Hao-Long Zeng
human	IHC	Transl Cancer Res; ALDH1B1 predicts poor survival for locally advanced nasopharyngeal carcinoma patients.; Authors - Tao Zhu