ALDH1L1 Polyclonal antibody

ALDH1L1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17390-1-AP

Synonyms

10 FTHFDH, ALDH1L1, FTHFD



Tested Applications

Positive WB detected inmouse liver tissue, mouse liver, rat liver
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17390-1-AP is ALDH1L1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1L1 fusion protein Ag11285
Full Name aldehyde dehydrogenase 1 family, member L1
Calculated molecular weight 99 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC027241
Gene symbol ALDH1L1
Gene ID (NCBI) 10840
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

Protocols

Product Specific Protocols
WB protocol for ALDH1L1 antibody 17390-1-APDownload protocol
IHC protocol for ALDH1L1 antibody 17390-1-APDownload protocol
IF protocol for ALDH1L1 antibody 17390-1-APDownload protocol
IP protocol for ALDH1L1 antibody 17390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Neurosci Bull

Expression Patterns of Inducible Cre Recombinase Driven by Differential Astrocyte-Specific Promoters in Transgenic Mouse Lines.

Authors - Neng-Yuan Hu
humanIHC,WB

NPJ Parkinsons Dis

Reduced astrocytic reactivity in human brains and midbrain organoids with PRKN mutations.

Authors - Masayoshi Kano
mouseIF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian