Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15090-1-AP targets ALDH3A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1687 Product name: Recombinant human ALDH3A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC002430 Sequence: MELEVRRVRQAFLSGRSRPLRFRLQQLEALRRMVQEREKDILTAIAADLCKSEFNVYSQEVITVLGEIDFMLENLPEWVTAKPVKKNVLTMLDEAYIQPQPLGVVLIIGAWNYPFVLTIQPLIGAIAAGNAVIIKPSELSENTAKILAKLLPQYLDQDLYIVINGGVEETTELLKQRFDHIFYTGNTAVGKIVMEAAAKHLTPVTLELGGKSPCYIDKDCDLDIVCRRITWGKYMNCGQTCIAPDYILCEASLQNQIVWKIKETVKEFYGENIKESPDYERIINLRHFKRILSLLEGQKIAFGGETDEATRYIA Predict reactive species |
| Full Name | aldehyde dehydrogenase 3 family, member A2 |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 50-57 kDa |
| GenBank Accession Number | BC002430 |
| Gene Symbol | ALDH3A2 |
| Gene ID (NCBI) | 224 |
| RRID | AB_2224316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51648 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| IHC protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| IP protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| WB protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALDH3A2 polyclonal antibody (Cat# 15090-1-AP) has 12 citations published across journals including Nature Neuroscience, Cell Death & Differentiation, J Biol Chem, J Transl Med, Transl Oncol, Int J Mol Sci, Cells, J Cell Mol Med, Funct Integr Genomics, Front Med, Am J Physiol, and Biol Proced Online. Research fields span neurodegeneration, cancer biology (colorectal, glioblastoma, prostate, renal, oral), lipid metabolism, inflammation, hepatic physiology, glaucoma, and ferroptosis.
| Species | Application | Title |
|---|---|---|
J Transl Med Deficiency of SDHC promotes metastasis by reprogramming fatty acid metabolism in colorectal cancer | ||
Cells Aldehyde Dehydrogenase, a Therapeutic Target in Chordoma: Analysis in 3D Cellular Models. | ||
Int J Mol Sci Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma | ||
Transl Oncol miR-1182-mediated ALDH3A2 inhibition affects lipid metabolism and progression in ccRCC by activating the PI3K-AKT pathway
| ||
Biol Proced Online Lipid Metabolic Rewiring During Continuous Prostate Cancer Progression Defines a Biologically Relevant Four-Gene Prognostic Signature. |
Reviews
What customers say
One reviewer (Elisabeth Storck, 2019) rated this antibody 3 out of 5 stars. She used it at a 1:1000 dilution in Western Blot on HeLa cells treated with control or ALDH3A2 siRNA for 72 hours. The review included an image showing the WB result, demonstrating the antibody's ability to detect ALDH3A2 knockdown. No additional written comments were provided beyond the experimental description.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Elisabeth (Verified Customer) (09-02-2019) | HeLa cells treated with control (Lane 1) or ALDH3A2 (Lane 2) siRNA for 72h.
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