ALDH3B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDH3B1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19446-1-AP

Synonyms

Aldehyde dehydrogenase 7, ALDH3B1, ALDH4, ALDH7

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 19446-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 19446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 19446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 19446-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 19446-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IHC detected in: human lung cancer tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 19446-1-AP is ALDH3B1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH3B1 fusion protein Ag7025
• Full Name: aldehyde dehydrogenase 3 family, member B1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC002553
• Gene Symbol: ALDH3B1
• Gene ID (NCBI): 221
• RRID: AB_2861362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH3B1(Aldehyde dehydrogenase family 3 member B1) is also named as ALDH7 and belongs to the aldehyde dehydrogenase family. It catalyzes fatty and aromatic aldehydes. ALDH3B1 may play a role in the detoxification of aldehydes produced during oxidative stress. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
human	IHC	Cell Death Dis; Aldehyde dehydrogenase 3B2 promotes the proliferation and invasion of cholangiocarcinoma by increasing Integrin Beta 1 expression; Authors - Yue Wang
human	WB	Front Immunol; The ALDH Family Contributes to Immunocyte Infiltration, Proliferation and Epithelial-Mesenchymal Transformation in Glioma.; Authors - Zeyu Wang ; KD Validated
mouse	WB	Cell Stress Chaperones; ALDH3B1 protects interfollicular epidermal cells against lipid peroxidation via the NRF2 pathway; Authors - Zhenjie Wu