ALDH6A1 Polyclonal antibody

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  WB analysis using 20452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 20452-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20452-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ALDH6A1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20452-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ALDH6A1, MMSADHA, MMSDH

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Tested Applications

• Positive WB detected in: rat liver tissue, mouse heart tissue, mouse kidney tissue, HEK-293 cells, rat kidney tissue, mouse liver tissue
• Positive IHC detected in: human liver tissue, human kidney tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:12000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

20452-1-AP targets ALDH6A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13879

  Product name: Recombinant human ALDH6A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 194-535 aa of BC004909

  Sequence: WMFPMAMVCGNTFLMKPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHEAVNFICDHPDIKAISFVGSNKAGEYIFERGSRHGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKKWLPELVEHAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRKIKVKGYENGNFVGPTIISNVKPNMTCYKEEIFGPVLVVLETETLDEAIQIVNNNPYGNGTAIFTTNGATARKYAHLVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSSPAVVMPTMGR

• Full Name: aldehyde dehydrogenase 6 family, member A1
• Calculated Molecular Weight: 535 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC004909
• Gene Symbol: ALDH6A1
• Gene ID (NCBI): 4329
• RRID: AB_10697664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02252
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancers (Basel); RNA Sequencing of Collecting Duct Renal Cell Carcinoma Suggests an Interaction between miRNA and Target Genes and a Predominance of Deregulated Solute Carrier Genes.; Authors - Sven Wach
human	WB	Front Neurol; Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.; Authors - Matthias Höllerhage
human	WB	Theranostics; DNMT1 mediates the disturbed flow-induced endothelial to mesenchymal transition through disrupting β-alanine and carnosine homeostasis; Authors - Jianan Zhao