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Alix Monoclonal antibody

Alix Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H9D9

Cat no : 67715-1-Ig

Synonyms

AIP1, ALG 2 interacting protein 1, Alix, ALIX; AIP1, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP



Tested Applications

Positive WB detected inHSC-T6 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 7 publications below

Product Information

67715-1-Ig targets Alix in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alix fusion protein Ag30437
Full Name programmed cell death 6 interacting protein
Calculated Molecular Weight 868 aa, 96 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC020066
Gene Symbol PDCD6IP
Gene ID (NCBI) 10015
RRIDAB_2882905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

Protocols

Product Specific Protocols
WB protocol for Alix antibody 67715-1-IgDownload protocol
IF protocol for Alix antibody 67715-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Biol Lett

Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway

Authors - Zhaoyi Liang
mouse,humanWB

J Thromb Haemost

Cancer-associated fibroblasts promote venous thrombosis through podoplanin/CLEC-2 interaction in podoplanin-negative lung cancer mouse model

Authors - Toshiaki Shirai
humanWB

Psychiatry Clin Neurosci

Human in vivo evidence of reduced astrocyte activation and neuroinflammation in patients with treatment-resistant depression following electroconvulsive therapy

Authors - Shu-Xian Xu
humanWB

J Microsc

A method for extraction of exosomes from breast tumour cells and characterisation by transmission electron microscopy

Authors - Zhiang Liu
humanWB

ACS Biomater Sci Eng

Enhancing Skin Injury Repair: Combined Application of PF-127 Hydrogel and hADSC-Exos Containing miR-148a-3p

Authors - Qiqi Zhang
humanWB

ACS Nano

Real-Time Dissection of the Exosome Pathway for Influenza Virus Infection

Authors - Jian Ao