Alix Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Alix Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9D9

Cat no : 67715-1-PBS

Synonyms

AIP1, ALG 2 interacting protein 1, Alix, ALIX; AIP1, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP

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Validation Data Gallery

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  WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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  WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67715-1-Ig (same clone as 67715-1-PBS)

  WB result of Alix antibody (67715-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67715-1-Ig (same clone as 67715-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Alix antibody (67715-1-Ig, Clone: 1H9D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67715-1-PBS targets Alix in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alix fusion protein Ag30437
• Full Name: programmed cell death 6 interacting protein
• Calculated Molecular Weight: 868 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC020066
• Gene Symbol: PDCD6IP
• Gene ID (NCBI): 10015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.