Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Calu-1 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16837-1-AP targets ALKBH5 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10516 Product name: Recombinant human ALKBH5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 57-383 aa of BC062339 Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG Predict reactive species |
| Full Name | alkB, alkylation repair homolog 5 (E. coli) |
| Calculated Molecular Weight | 458 aa, 51 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC062339 |
| Gene Symbol | ALKBH5 |
| Gene ID (NCBI) | 54890 |
| RRID | AB_2242665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P6C2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALKBH5 antibody 16837-1-AP | Download protocol |
| IP protocol for ALKBH5 antibody 16837-1-AP | Download protocol |
| WB protocol for ALKBH5 antibody 16837-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALKBH5 Polyclonal antibody (Cat# 16837-1-AP) has 271 total citations. Publications appear in high-impact journals including Nature Communications, Nature Cell Biology, Nature Genetics, Cell Research, Molecular Cell, Science Advances, PNAS, Cell Death & Disease, and Signal Transduction and Targeted Therapy. Research fields encompass m6A RNA epitranscriptomics, oncology (hepatocellular, colorectal, glioblastoma, ovarian, cervical cancers), cardiovascular disease, neurology, immunology, fibrosis, metabolic disorders, and infectious diseases. Primary applications include WB, RIP, IHC, and IF across human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Characterization of ACTN4 as a novel antiviral target against SARS-CoV-2 | ||
Mol Cancer Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification. | ||
Cancer Commun (Lond) Targeting histone deacetylase suppresses tumor growth through eliciting METTL14-modified m6 A RNA methylation in ocular melanoma | ||
Nat Cancer A lactate-α-ketoglutarate metabolic circuit in tumor-infiltrating regulatory T cells accelerates tumor progression by inducing NK cell senescence |
Reviews
What customers say
Most reviewers report satisfactory performance for Western blot and IHC applications. Two users confirmed clear detection in H9C2 cells with positive results. One user noted some non-specific bands in colorectal cancer cells but found the antibody acceptable for ALKBH5 detection with siRNA validation. IHC staining in mouse kidney showed good proximal tubule localization. However, one reviewer reported failure to detect knockdown in RCC cell lines, noting that an alternative antibody from a different vendor showed clearer results. Overall, the antibody performs well in most applications, though some users may encounter non-specific signals or validation challenges depending on the cell type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
PK (Verified Customer) (07-08-2022) | GOOD
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SU (Verified Customer) (03-22-2022) |
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ANIRBAN (Verified Customer) (04-07-2021) | Tried to validate the antibody (AlkBH5) in two different cell lines (786-o, HK2) using three siRNA constructs from IDT. Using the Proteintech antibody, I didn't see any evedence of KD in the lysates (see the figure attached). When I used the Cell Signaling antibody for AlkBH5 (#80283) I observed a clear evedence of KD in the same lysates that I mentioned before. scr=scrambled; si-1,2,3=siRNAs targeting AlkBH5.
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Bill (Verified Customer) (12-23-2019) | This antibody has some unspecific band, but is ok to detect ALKBH5, siRNA validated.
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Daniel (Verified Customer) (12-04-2019) | Staining of kidney proximal tubules
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