ALKBH5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALKBH5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

149

Reviews (5)

Cat no : 16837-1-AP

Synonyms

ABH5, ALKBH5, OFOXD, OFOXD1

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Validation Data Gallery

• 

  WB analysis using 16837-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16837-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16837-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human testis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 16 publications below
• WB: See 135 publications below
• IHC: See 17 publications below
• IF: See 10 publications below
• CoIP: See 1 publications below
• RIP: See 10 publications below

Product Information

16837-1-AP targets ALKBH5 in WB, IHC, IF, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALKBH5 fusion protein Ag10516
• Full Name: alkB, alkylation repair homolog 5 (E. coli)
• Calculated Molecular Weight: 458 aa, 51 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC062339
• Gene Symbol: ALKBH5
• Gene ID (NCBI): 54890
• RRID: AB_2242665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Publications

Species	Application	Title
human	WB	Nat Genet; N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.; Authors - Yuan Li
human	IF	Cell Res; The m6A methylome of SARS-CoV-2 in host cells.; Authors - Jun'e Liu ; KD Validated
mouse	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human	WB	Nat Commun; Circular RNA encoded MET variant promotes glioblastoma tumorigenesis; Authors - Jian Zhong
human,mouse	WB	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
mouse	WB	Nat Commun; ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice; Authors - Tao Zhuang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PK (Verified Customer) (07-08-2022)

GOOD

• Applications: Western Blot
• Cell Tissue Type: H9C2

ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)

FH

SU (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: H9C2

ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)

FH

ANIRBAN (Verified Customer) (04-07-2021)

Tried to validate the antibody (AlkBH5) in two different cell lines (786-o, HK2) using three siRNA constructs from IDT. Using the Proteintech antibody, I didn't see any evedence of KD in the lysates (see the figure attached). When I used the Cell Signaling antibody for AlkBH5 (#80283) I observed a clear evedence of KD in the same lysates that I mentioned before. scr=scrambled; si-1,2,3=siRNAs targeting AlkBH5.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2K
• Cell Tissue Type: RCC cell lines

ALKBH5 Antibody Western Blot validation (1:2K dilution) in RCC cell lines (Cat no:16837-1-AP)

FH

Bill (Verified Customer) (12-23-2019)

This antibody has some unspecific band, but is ok to detect ALKBH5, siRNA validated.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colerectal cancer cells

FH

Daniel (Verified Customer) (12-04-2019)

Staining of kidney proximal tubules

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney (TRACK model)

ALKBH5 Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse kidney (TRACK model) (Cat no:16837-1-AP)

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