Tested Applications
| Positive WB detected in | 4T1 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67811-1-Ig targets ALKBH5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, hamster |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10490 Product name: Recombinant human ALKBH5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 57-383 aa of BC062339 Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG Predict reactive species |
| Full Name | alkB, alkylation repair homolog 5 (E. coli) |
| Calculated Molecular Weight | 458 aa, 51 kDa |
| Observed Molecular Weight | 40-44 kDa |
| GenBank Accession Number | BC062339 |
| Gene Symbol | ALKBH5 |
| Gene ID (NCBI) | 54890 |
| RRID | AB_2918574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6P6C2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALKBH5 antibody 67811-1-Ig | Download protocol |
| WB protocol for ALKBH5 antibody 67811-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALKBH5 antibody (Cat# 67811-1-Ig) has 19 citations across journals including Science, Nature Communications, Journal of Advanced Research, Advanced Science, Acta Pharmacologica Sinica, Apoptosis, Cell Molecular Life Sciences, Cell Biosciences, Acta Physiologica, Scientific Reports, Cell Signaling, FASEB Journal, Genes & Immunity, Viruses, Drug Metabolism & Disposition, Applied Biochemistry and Biotechnology, and Neuroreport. Research fields encompass m6A RNA methylation, hepatic metabolism, neuroprotection, oncology, pulmonary fibrosis, ferroptosis, and viral replication.
| Species | Application | Title |
|---|---|---|
Science Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling
| ||
J Adv Res Targeting myofibroblast copper vulnerability reverses pulmonary fibrosis via METTL3-directed STAT6 m6A driving cuproptosis. | ||
Adv Sci (Weinh) m(6)A-Mediated Glycolysis by IL-37 Drives T Cell Metabolic Reprogramming to Regulate Colitis. | ||
Acta Pharmacol Sin Circular RNA circDhx32 promotes cardiac inflammatory responses in mouse cardiac ischemia-reperfusion injury via binding to FOXO1 competed with AdipoR1 | ||
Apoptosis NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer |
Reviews
What customers say
One reviewer (5/5 stars) tested this ALKBH5 antibody by Western Blot on human lung cancer cell lines (Ludlu-1, H1299, A459) at a 1:1000 dilution. They reported that while the band was faint after overnight incubation at 4°C, it was still their best detection result for ALKBH5 so far, and they recommend the antibody.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nikolett (Verified Customer) (09-05-2025) | Antibody works on human lung cancer cell lines as 1:1000 but I only saw a faint band after overnight incubation at 4°C. So far my best detection for ALKBH5. I recommend this antibody.
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