ALKBH5 Monoclonal antibody, PBS Only

• 

  WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)

  WB result of ALKBH5 antibody (67811-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67811-1-Ig (same clone as 67811-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67811-1-Ig (same clone as 67811-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67811-1-Ig (same clone as 67811-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ALKBH5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67811-1-PBS

Clone No.2D2B2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

---

Product Information

67811-1-PBS targets ALKBH5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10490

  Product name: Recombinant human ALKBH5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 57-383 aa of BC062339

  Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG

• Full Name: alkB, alkylation repair homolog 5 (E. coli)
• Calculated Molecular Weight: 458 aa, 51 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: BC062339
• Gene Symbol: ALKBH5
• Gene ID (NCBI): 54890
• RRID: AB_2918574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6P6C2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.