ALPL Recombinant monoclonal antibody

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• ALPL Recombinant monoclonal antibody (82915-2-RR)

• 

  WB analysis of L02 using 82915-2-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 82915-2-RR (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 82915-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALPL Recombinant antibody (82915-2-RR, Clone:230212D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

ALPL Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 82915-2-RR

Clone No.230212D5

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Alkaline phosphatase, 230212D5, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme

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Tested Applications

• Positive WB detected in: L02 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

82915-2-RR targets ALPL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1664

  Product name: Recombinant human ALPL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 23-330 aa of BC021289

  Sequence: EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLL

• Full Name: alkaline phosphatase, liver/bone/kidney
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC021289
• Gene Symbol: ALPL
• Gene ID (NCBI): 249
• RRID: AB_3670648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P05186
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is expressed in multiple tissues including liver, bone, and kidney, rather than being restricted to a single tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	bioRxiv; A Novel FNDC1-NAMPT-NAD axis is Implicated in Small and Large-vessel Arterial Disease and Drives Vascular Calcification.; Authors - Sujin Lee