ALPP Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP02613-1

  Sandwich ELISA standard curve of MP02613-1, Human PLAP Recombinant Matched Antibody Pair - PBS only. 86752-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3065. 86752-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02613-1

  Plasma of five individual healthy human donors was measured. The human PLAP concentration of detected samples was determined to be 0.22 ng/mL with a range of 0.09 - 0.52 ng/mL.

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  Sample test of MP02613-1

  The mean PLAP concentration was determined to be 5.44 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 37.23 ng/mL in human placenta tissue extract based on a 3.8 mg/mL extract load.

ALPP Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86752-1-PBS

Clone No.251261A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

PLAP, ALP, EC:3.1.3.1, PALP, PLAP-1

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Product Information

86752-1-PBS targets ALPP as part of a matched antibody pair:

MP02613-1: 86752-2-PBS capture and 86752-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3065

  Product name: Recombinant Human PLAP protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 23-506 aa of NM_001632.4

  Sequence: IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD

• Full Name: alkaline phosphatase, placental (Regan isozyme)
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: NM_001632.4
• Gene Symbol: PLAP
• Gene ID (NCBI): 250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05187
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.