AMD1 Polyclonal antibody

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• AMD1 Polyclonal antibody (11052-1-AP)

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• KD/KO Validated

AMD1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11052-1-AP Copied!

Publications (23)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

ADOMETDC, AMD, AMD1, SAMDC

• 

  WB analysis of A431 using 11052-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11052-1-AP (AMD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 11052-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11052-1-AP (AMD1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 11052-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11052-1-AP (AMD1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 11052-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11052-1-AP (AMD1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11052-1-AP targets AMD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1525

  Product name: Recombinant human AMD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-334 aa of BC000171

  Sequence: MEAAHFFEGTEKLLEVWFSRQQPDANQGSGDLRTIPRSEWDILLKDVQCSIISVTKTDKQEAYVLSESSMFVSKRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAIFPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKFVTTLFVNQSSKCRTVLASPQKIEGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS

• Full Name: adenosylmethionine decarboxylase 1
• Calculated Molecular Weight: 38 kDa, 32 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC000171
• Gene Symbol: AMD1
• Gene ID (NCBI): 262
• RRID: AB_2226430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17707
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The antibody (Cat# 11052-1-AP) has 23 total citations spanning journals including Nature, Molecular Cancer, Nature Metabolism, Cancer Research, Nucleic Acids Research, Cancer Letters, Clinical and Translational Medicine, Breast Cancer Research, Cells, International Journal of Molecular Science, International Journal of Cancer, Scientific Reports, iScience, Carcinogenesis, Experimental Cell Research, BMC Pharmacology and Toxicology, Biology of Reproduction, medRxiv, and Heliyon. Research fields cover polyamine metabolism, cancer (prostate, breast, gastric, melanoma, leukemia), ferroptosis, cellular senescence, and metabolic regulation.

Automatically generated from the 23 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Polyamine-dependent metabolic shielding regulates alternative splicing.; Authors - Maria Ponce-Rodriguez ; KD Validated
human	WB,IHC	Nature; mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer.; Authors - Amaia Zabala-Letona ; KD Validated
mouse	WB	Nature; Spermine is an endogenous iron chelator that inhibits ferroptosis; Authors - Man Li
human	WB	Mol Cancer; Polyamine and EIF5A hypusination downstream of c-Myc confers targeted therapy resistance in BRAF mutant melanoma; Authors - Byung-Sun Park
human	WB	Nat Metab; Polyamine pathway activity promotes cysteine essentiality in cancer cells.; Authors - Tong Zhang
mouse	WB	Nat Metab; Angiocrine polyamine production regulates adiposity; Authors - Erika Monelli