AMH Polyclonal antibody

AMH Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23479-1-AP

Synonyms

AMH, anti Mullerian hormone, MIF, MIS

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Validation Data Gallery

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  IHC staining of human ovary using 23479-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 23479-1-AP (AMH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 23479-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 23479-1-AP (AMH Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat ovary using 23479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 23479-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 23479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 23479-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 23479-1-AP

  Immunofluorescent analysis of HeLa cells using 23479-1-AP (AMH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human ovary tissue, rat ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 23479-1-AP is AMH Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMH fusion protein Ag20166
• Full Name: anti-Mullerian hormone
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: BC049194
• Gene Symbol: AMH
• Gene ID (NCBI): 268
• RRID: AB_2879285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a dimeric glycoprotein and a member of the TGF-β family. AMH, produced by the Sertoli cells of the fetal testis, induces the regression of the Müllerian ducts, the anlagen of the female reproductive tract. However, after birth, this sex-dimorphic expression pattern is lost and AMH is also expressed in granulosa cells of growing follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve.

Publications

Species	Application	Title
human	IF	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao