Tested Applications
| Positive IHC detected in | human ovary tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse ovary tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23479-1-AP targets AMH in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20166 Product name: Recombinant human AMH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-560 aa of BC049194 Sequence: MRDLPLTSLALVLSALGALLGTEALRAEEPAVGTSGLIFREDLDWPPGSPQEPLCLVALGGDSNGSSSPLRVVGALSAYEQAFLGAVQRARWGPRDLATFGVCNTGDRQAALPSLRRLGAWLRDPGGQRLVVLHLEEVTWEPTPSLRFQEPPPGGAGPPELALLVLYPGPGPEVTVTRAGLPGAQSLCPSRDTRYLVLAVDRPAGAWRGSGLALTLQPRGEDSRLSTARLQALLFGDDHRCFTRMTPALLLLPRSEPAPLPAHGQLDTVPFPPPRPSAELEESPPSADPFLETLTRLVRALRVPPARASAPRLALDPDALAGFPQGLVNLSDPAALERLLDGEEPLLLLLRPTAATTGDPAPLHDPTSAPWATALARRVAAELQAAAAELRSLPGLPPATAPLLARLLALCPGGPGGLGDPLRALLLLKALQGLRVEWRGRDPRGPGRAQRSAGATAADGPCALRELSVDLRAERSVLIPETYQANNCQGVCGWPQSDRNPRYGNHVVLLLKMQARGAALARPPCCVPTAYAGKLLISLSEERISAHHVPNMVATECGCR Predict reactive species |
| Full Name | anti-Mullerian hormone |
| Calculated Molecular Weight | 59 kDa |
| GenBank Accession Number | BC049194 |
| Gene Symbol | AMH |
| Gene ID (NCBI) | 268 |
| RRID | AB_2879285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P03971 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a dimeric glycoprotein and a member of the TGF-β family. AMH, produced by the Sertoli cells of the fetal testis, induces the regression of the Müllerian ducts, the anlagen of the female reproductive tract. However, after birth, this sex-dimorphic expression pattern is lost and AMH is also expressed in granulosa cells of growing follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AMH antibody 23479-1-AP | Download protocol |
| IHC protocol for AMH antibody 23479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AMH Polyclonal antibody (Cat# 23479-1-AP) has 2 citations. They appear in ACS Appl Mater Interfaces and Mol Hum Reprod. The research fields are reproductive biology and ovarian physiology—one study investigates a 3D follicle culture system reducing oxidative stress in human granulosa cells, while the other examines S14G-Humanin's protective effect against cyclophosphamide-induced premature ovarian insufficiency in mice. Both citations used the antibody for immunofluorescence (IF).
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells | ||
Mol Hum Reprod S14G-Humanin ameliorates ovarian dysfunction in a cyclophosphamide-induced premature ovarian insufficiency mouse model. |
