JAML Polyclonal antibody

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• JAML Polyclonal antibody (21302-1-AP)

JAML Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21302-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Gm638, Dendritic cell-specific protein CREA7-1, CREA7 4, CREA7 1, AMICA1

• 

  WB analysis using 21302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21302-1-AP (JAML antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 21302-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21302-1-AP (JAML antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 21302-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21302-1-AP (JAML antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells, Jurkat cells, L02 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21302-1-AP targets JAML in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15900

  Product name: Recombinant human AMICA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 40-275 aa of BC100797

  Sequence: VPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRTLVTPAALRPLVLGGNQLVIIVGIVCATILLLPVLILIVKKTCGNKSSVNSTVLVKN

• Full Name: adhesion molecule, interacts with CXADR antigen 1
• Calculated Molecular Weight: 394 aa, 44 kDa
• Observed Molecular Weight: 44 kDa, 50-70 kDa
• GenBank Accession Number: BC100797
• Gene Symbol: JAML
• Gene ID (NCBI): 120425
• RRID: AB_10733103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86YT9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Junctional adhesion molecules (JAM-A, JAM-B, and JAM-C) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites (PMID: 19064666). JAML (junctional adhesion molecule-like), also known as AMICA1, is related to JAM proteins. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML is expressed on leukocytes and promotes their adhesion to endothelial cells (PMID: 12869515). JAML can regulate neutrophil transepithelial migration via binding interactions with epithelial coxsackie and adenovirus receptor (CAR) (PMID: 15800062). The full-length human JAML protein has a calculated molecular weight of 44 kDa. This antibody raised against 40-275 aa of human JAML protein detects bands with molecular wight of 44 kDa, and 50-70 kDa which probably represent different glycoforms of the protein (PMID: 12869515; 24621992; 15800062).

Publications

What published studies show

The JAML Polyclonal antibody (Cat# 21302-1-AP) has 5 citations published in Cancer Letters, Journal of Translational Medicine, Clinical Science, Frontiers in Immunology, and European Journal of Pharmacology. Research fields span oncology (colorectal cancer, lung adenocarcinoma, endometrial cancer), cardiovascular disease (atherosclerosis), and metabolic disease (diabetic kidney injury), with applications in WB, IHC, and IF.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Cancer Lett; Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer; Authors - Minghua Bai
human	IHC,IF	J Transl Med; Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma.; Authors - Qian Wu
mouse	IHC,IF	Clin Sci (Lond); Silencing of junctional adhesion molecule-like protein attenuates atherogenesis and enhances plaque stability in ApoE-/- mice.; Authors - Yu Sun
human	WB,IHC	Front Immunol; The prognostic significance of JAML and its role in remodeling the immune microenvironment via the cGAS-STING pathway in endometrial cancer.; Authors - Chenfan Tian ; KD Validated
mouse	WB,IHC	Eur J Pharmacol; Resveratrol ameliorates diabetic kidney injury by reducing lipotoxicity and modulates expression of components of the junctional adhesion molecule-like/sirtuin 1 lipid metabolism pathway.; Authors - Wei Gu