AMZ1 Polyclonal antibody

AMZ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24484-1-AP

Synonyms

KIAA1950, EC:3.4.-.-, Archeobacterial metalloproteinase-like protein 1, Archaemetzincin-1, Archaemetzincin 1

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 24484-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 24484-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24484-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24484-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24484-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 24484-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 24484-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 24484-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMZ1 antibody (24484-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24484-1-AP targets AMZ1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMZ1 fusion protein Ag20248
• Full Name: archaelysin family metallopeptidase 1
• Calculated Molecular Weight: 498 aa, 55 kDa
• Observed Molecular Weight: 33 kDa, 28 kDa
• GenBank Accession Number: BC146753
• Gene Symbol: AMZ1
• Gene ID (NCBI): 155185
• RRID: AB_2879565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMZ1, also named as KIAA1950, is a new human Zinc metalloproteases. It exhibits aminopeptidase activity against neurogranin in vitro. AMZ1 does not hydrolyze angiotensin-2. It is mainly detected in liver and heart. AMZ1 has some isoforms with MW 55 kDa, 33 kDa, 30 kDa and 28 kDa.