ANAPC7 Polyclonal antibody, PBS Only

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• ANAPC7 Polyclonal antibody, PBS Only (21761-1-PBS)

ANAPC7 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21761-1-PBS Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ANAPC 7, APC7

• 

  WB analysis using 21761-1-AP (same clone as 21761-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21761-1-AP (ANAPC7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21761-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21761-1-AP (same clone as 21761-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21761-1-AP (ANAPC7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21761-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 21761-1-AP (same clone as 21761-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21761-1-AP (ANAPC7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21761-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 21761-1-AP (same clone as 21761-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 21761-1-AP (ANAPC7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21761-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 21761-1-AP (same clone as 21761-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 21761-1-AP (ANAPC7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21761-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

21761-1-PBS targets ANAPC7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16339

  Product name: Recombinant human ANAPC7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 264-565 aa of BC141848

  Sequence: QMLDPYLIKGMDVYGYLLAREGRLEDVENLGCRLFNISDQHAEPWVVSGCHSFYSKRYSRALYLGAKAIQLNSNSVQALLLKGAALRNMGRVQEAIIHFREAIRLAPCRLDCYEGLIECYLASNSIREAMVIANNVYKTLGANAQTLTLLATVCLEDPVTQEKAKTLLDKALTQRPDYIKAVVKKAELLSREQKYEDGIALLRNALANQSDCVLHRILGDFLVAVNEYQEAMDQYSIALSLDPNDQKSLEGMQKMEKEESPTDATQEEDVDDMEGSGEEGDLEGSDSEAAQWADQEQWFGMQ

• Full Name: anaphase promoting complex subunit 7
• Calculated Molecular Weight: 565 aa, 63 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC141848
• Gene Symbol: ANAPC7
• Gene ID (NCBI): 51434
• RRID: AB_3669373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJX3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ANAPC7 Involved in brain development through the specific ubiquitination and clearance of MKI67 from constitutive heterochromatin after neuronal progenitors exit mitosis.