ANK3 Polyclonal antibody

ANK3 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27766-1-AP

Synonyms

ANK 3, ANK3, Ankyrin 3, ANKYRIN G

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Validation Data Gallery

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  IHC staining of mouse brain using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 27766-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 27766-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27766-1-AP targets ANK3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANK3 fusion protein Ag26948
• Full Name: ankyrin 3, node of Ranvier (ankyrin G)
• Calculated Molecular Weight: 480 kDa
• Observed Molecular Weight: 204 kDa
• GenBank Accession Number: NM_001149
• Gene Symbol: ANK3
• Gene ID (NCBI): 288
• RRID: AB_2880966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.