Filter: AllIHCIF-P

IHC staining of mouse brain using 27766-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 27766-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 27766-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 27766-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 27766-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 27766-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 27766-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 27766-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 27766-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANK3 Polyclonal antibody

Name: ANK3 Polyclonal antibody
Brand: Proteintech
SKU: 27766-1-AP

ANK3 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 27766-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

ANK 3, Ankyrin 3, ANKYRIN G, Ankyrin3, ANKYRING

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	mouse brain tissue, human kidney tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27766-1-AP targets ANK3 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26948 Product name: Recombinant human ANK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3668-3870 aa of NM_001149 Sequence: METNLERNVETPTVEPNPSIPTSGECQEGTSSSGSLEKSAAATNTSKVDPKLRTPIKMGISASTMTMKKEGPGEITDKIEAVMTSCQGLENETITMISNTANSQMGVRPHEKHDFQKDNFNNNNNLDSSTIQTDNIMSNIVLTEHSAPTCTTEKDNPVKVSSGKKTGVLQGHCVRDKQKVLGEQQKTKELIGIRQKSKLPIKAT Predict reactive species
Full Name	ankyrin 3, node of Ranvier (ankyrin G)
Calculated Molecular Weight	480 kDa
Observed Molecular Weight	204 kDa
GenBank Accession Number	NM_001149
Gene Symbol	ANK3
Gene ID (NCBI)	288
RRID	AB_2880966
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12955
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.