ANKLE1 Polyclonal antibody

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• ANKLE1 Polyclonal antibody (24080-1-AP)

ANKLE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24080-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ANKRD41, Ankyrin repeat and LEM domain-containing protein 1, Ankyrin repeat domain-containing protein 41, EC:3.1.-.-, LEM3

• 

  WB analysis of HeLa using 24080-1-AP

  Hela cells were subjected to SDS PAGE followed by western blot with 24080-1-AP (ANKLE1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 24080-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24080-1-AP (ANKLE1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 24080-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24080-1-AP (ANKLE1 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 24080-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24080-1-AP (ANKLE1 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Hela cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24080-1-AP targets ANKLE1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21253

  Product name: Recombinant human ANKLE1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 232-579 aa of BC125274

  Sequence: SLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPPPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGPEFSGHSLELAAALRTGCIPDVQADEDTLAQQFERPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG

• Full Name: ankyrin repeat and LEM domain containing 1
• Calculated Molecular Weight: 615 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC125274
• Gene Symbol: ANKLE1
• Gene ID (NCBI): 126549
• RRID: AB_2879428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8NAG6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The ANKLE1 Polyclonal antibody (Cat# 24080-1-AP) has 1 total citation. It appears in the World Journal of Surgical Oncology (IF 2.8), published in June 2022. The cited study performed a collective analysis of LEM-domain protein expression and prognosis in prostate cancer, utilizing this antibody for Western blot and immunohistochemistry applications in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	World J Surg Oncol; Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.; Authors - Tianzhen He