ANKLE1 Polyclonal antibody

ANKLE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24080-1-AP

Synonyms

ANKLE1, ANKRD41, LEM3, LEMD6

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Validation Data Gallery

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  WB analysis of HeLa using 24080-1-AP

  Hela cells were subjected to SDS PAGE followed by western blot with 24080-1-AP (ANKLE1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24080-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24080-1-AP (ANKLE1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24080-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24080-1-AP (ANKLE1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 24080-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24080-1-AP (ANKLE1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 24080-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24080-1-AP (ANKLE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Hela cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 24080-1-AP is ANKLE1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKLE1 fusion protein Ag21253
• Full Name: ankyrin repeat and LEM domain containing 1
• Calculated Molecular Weight: 615 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC125274
• Gene Symbol: ANKLE1
• Gene ID (NCBI): 126549
• RRID: AB_2879428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	World J Surg Oncol; Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.; Authors - Tianzhen He