ANKRD6 Recombinant monoclonal antibody

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  IHC staining of human lung cancer using 84210-4-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84210-4-RR (ANKRD6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 84210-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using ANKRD6 antibody (84210-4-RR, Clone: 241494D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84210-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84210-4-RR against Human ANKRD6 were performed. The affinity constant is 0.722 nM.

ANKRD6 Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 84210-4-RR

Clone No.241494D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

241494D11, ANKRD 6, Ankyrin repeat domain-containing protein 6, Diversin

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Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84210-4-RR targets ANKRD6 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19328

  Product name: Recombinant human ANKRD6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-260 aa of BC042173

  Sequence: MSQQDAVAALSERLLVAAYKGQTENVVQLINKGARVAVTKHGRTPLHLAANKGHLPVVQILLKAGCDLDVQDDGDQTALHRATVVGNTEIIAALIHEGCALDRQDKDGNTALHEASWHGFSQSAKLLVKAGANVLAKNKAGNTALHLACQNSHSQSTRVLLLAGSRADLKNNAGDTCLHVAARYNHLSIIRLLLTAFCSVHEKNQAGDTALHVAAALNHKKVAKILLEAGADTTIVNNAGQTPLETARYHNNPEVALLLT

• Full Name: ankyrin repeat domain 6
• Calculated Molecular Weight: 727 aa, 80 kDa
• GenBank Accession Number: BC042173
• Gene Symbol: ANKRD6
• Gene ID (NCBI): 22881
• RRID: AB_3671767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y2G4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.