IHC staining of human lung cancer using 84210-4-RR
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84210-4-RR (ANKRD6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84210-4-RR (ANKRD6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 84210-4-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using ANKRD6 antibody (84210-4-RR, Clone: 241494D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using ANKRD6 antibody (84210-4-RR, Clone: 241494D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Affinity and Kinetic Characterization of 84210-4-RR
Biolayer interferometry (BLl) kinetic assays of 84210-4-RR
against Human ANKRD6 were performed. The affinity constant is 0.722 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A549 cells
Recommended dilution
Application
Dilution
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
84210-4-RR targets ANKRD6 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
IHC staining of human lung cancer using 84210-4-RR
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84210-4-RR (ANKRD6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A549 using 84210-4-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using ANKRD6 antibody (84210-4-RR, Clone: 241494D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
AFFINITY Figures
Affinity and Kinetic Characterization of 84210-4-RR
Biolayer interferometry (BLl) kinetic assays of 84210-4-RR
against Human ANKRD6 were performed. The affinity constant is 0.722 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ANKRD6 Recombinant antibody
Catalog Number
84210-4-RR
Citations
-
Dilutions
IHC : 1:200-1:800 IF/ICC : 1:125-1:500
Applications
IHC, IF/ICC, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
