Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, rat brain tissue, mouse brain tissue, HEK-293 cells, U-251 cells, PC-12 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66643-1-Ig targets ANLN in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19294 Product name: Recombinant human ANLN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 818-1124 aa of BC034692 Sequence: VCSTVQKPDAANYYYLIILKAGAENMVATPLASTSNSLNGDALTFTTTFTLQDVSNDFEINIEVYSLVQKKDPSGLDKKKKTSKSKAITPKRLLTSITTKSNIHSSVMASPGGLSAVRTSNFALVGSYTLSLSSVGNTKFVLDKVPFLSSLEGHIYLKIKCQVNSSVEERGFLTIFEDVSGFGAWHRRWCVLSGNCISYWTYPDDEKRKNPIGRINLANCTSRQIEPANREFCARRNTFELITVRPQREDDRETLVSQCRDTLCVTKNWLSADTKEERDLWMQKLNQVLVDIRLWQPDACYKPIGKP Predict reactive species |
| Full Name | anillin, actin binding protein |
| Calculated Molecular Weight | 1124 aa, 124 kDa |
| Observed Molecular Weight | 110 kDa~120 kDa |
| GenBank Accession Number | BC034692 |
| Gene Symbol | ANLN |
| Gene ID (NCBI) | 54443 |
| RRID | AB_2815000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NQW6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Anillin, an actin-binding protein, is a conserved cleavage furrow component important for cytokinesis. The human anillin protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. It interactions with at least two other furrow proteins, actin and the septins through its C-terminal PH domain and N-terminal actin-binding domain. In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ingression. Anillin has been found to be overexpressed in tumors and may serve as promising marker for pancreatic carcinoma molecular diagnostics as well as a prognostic indicator for non-small cell lung cancers.
Publications
What published studies show
The ANLN antibody (Cat# 66643-1-Ig) has 8 total citations published in journals including Molecular Cell, Biofactors, Cancers (Basel), Molecular and Cellular Biochemistry, Cell Cycle, Pathology Research and Practice, Frontiers in Genetics, and Clinical and Experimental Pharmacology and Physiology. These citations span research fields such as cancer biology (renal, gallbladder, pancreatic, hepatic, lung, and breast cancers), cell cycle regulation, RNA metabolism, and immunotherapy response prediction.
| Species | Application | Title |
|---|---|---|
Mol Cell The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance | ||
Biofactors The Immunotherapeutic Target ANLN Promotes Renal Clear Cell Carcinoma by Regulating PI3K/Akt and P53 Signaling Pathways
| ||
Cancers (Basel) ANLN Promotes the Proliferation and Migration of Gallbladder Cancer Cells via STRA6-Mediated Activation of PI3K/AKT Signaling
| ||
Mol Cell Biochem PUS7 promotes the progression of pancreatic cancer by interacting ANLN to activate MYC pathway | ||
Cell Cycle CDK1-PLK1/SGOL2/ANLN pathway mediating abnormal cell division in cell cycle may be a critical process in hepatocellular carcinoma. | ||
Pathol Res Pract Vasculogenic mimicry-associated novel gene signature predicted prognosis and response to immunotherapy in lung adenocarcinoma |
