ANO2 Monoclonal antibody, PBS Only

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  WB analysis using 67638-1-Ig (same clone as 67638-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 67638-1-Ig (same clone as 67638-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using ANO2 antibody (67638-1-Ig, Clone: 2F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

ANO2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67638-1-PBS

Clone No.2F12G1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B

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Product Information

67638-1-PBS targets ANO2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: anoctamin 2
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: NM_020373
• Gene Symbol: ANO2
• Gene ID (NCBI): 57101
• RRID: AB_2882839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQ90
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.