ANO2 Monoclonal antibody

ANO2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2F12G1

Cat no : 67638-1-Ig

Synonyms

ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B

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Validation Data Gallery

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  WB analysis using 67638-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 67638-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 67638-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 67638-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 67638-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using ANO2 antibody (67638-1-Ig, Clone: 2F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Y79 cells, NCCIT cells, U2OS cells, MDA-MB-231 cells, T-47D cells, HeLa cells
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67638-1-Ig targets ANO2 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: anoctamin 2
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: NM_020373
• Gene Symbol: ANO2
• Gene ID (NCBI): 57101
• RRID: AB_2882839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.