ANP32CP Recombinant antibody

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  WB analysis using 84553-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84553-1-RR (ANP32CP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of LNCaP using 84553-1-RR

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using ANP32CP antibody (84553-1-RR, Clone: 241562D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84553-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84553-1-RR against Human ANP32CP were performed. The affinity constant is 7.76 nM.

ANP32CP Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84553-1-RR

Clone No.241562D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ANP32C, Tumorigenic protein pp32r1, PP32R1, 241562D12

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Tested Applications

• Positive WB detected in: HUVEC cells, BxPC-3 cells, LNCaP cells, MCF-7 cells, DU 145 cells
• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84553-1-RR targets ANP32CP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ANP32CP fusion protein Ag35293
• Full Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: NM_012403.1
• Gene Symbol: ANP32C/PP32R1
• Gene ID (NCBI): 23520
• RRID: AB_3672061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43423
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.