Validation Data Gallery
|Positive WB detected in||HepG2 cells, human liver tissue, human kidney tissue, PC-3 cells, THP-1 cells, mouse kidney tissue, mouse liver tissue|
|Positive IHC detected in||human tonsillitis tissue, human colon tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse kidney tissue|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14553-1-AP targets CD13 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD13 fusion protein Ag5976|
|Full Name||alanyl (membrane) aminopeptidase|
|Calculated molecular weight||110 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||BC058928|
|Gene ID (NCBI)||290|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)
|Product Specific Protocols|
|WB protocol for CD13 antibody 14553-1-AP||Download protocol|
|IHC protocol for CD13 antibody 14553-1-AP||Download protocol|
|IF protocol for CD13 antibody 14553-1-AP||Download protocol|
|FC protocol for CD13 antibody 14553-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Rapid Screening of Peptide Probes through in situ Single Bead Sequencing Microarray.
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