Tested Applications
| Positive WB detected in | U-937 cells |
| Positive IHC detected in | human kidney tissue, human tonsillitis tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue, human kidney tissue |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66211-1-Ig targets CD13 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5976 Product name: Recombinant human ANPEP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 609-967 aa of BC058928 Sequence: NDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK Predict reactive species |
| Full Name | alanyl (membrane) aminopeptidase |
| Calculated Molecular Weight | 110 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC058928 |
| Gene Symbol | CD13 |
| Gene ID (NCBI) | 290 |
| ENSEMBL Gene ID | ENSG00000166825 |
| RRID | AB_2881602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15144 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M-1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD13 antibody 66211-1-Ig | Download protocol |
| IHC protocol for CD13 antibody 66211-1-Ig | Download protocol |
| WB protocol for CD13 antibody 66211-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CD13 Monoclonal antibody (2D8D11), catalog number 66211-1-Ig, has 12 citations spanning journals including Cell Stem Cell, Theranostics, Alzheimer's & Dementia, Journal of Translational Medicine, Pharmacetics, Molecular & Cellular Proteomics, Scientific Reports, Cancer Management Research, Translational Cancer Research, Biological & Pharmaceutical Bulletin, bioRxiv, and Research Square. Research fields cover hepatocellular carcinoma, cancer stem cells, Alzheimer's disease, head and neck squamous cell carcinoma, mTORC1 signaling, pancreatic cancer, acute pancreatitis, VHL-related pathology, and cognitive decline.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Human airway submucosal gland organoids to study respiratory inflammation and infection | ||
Theranostics RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog. | ||
Alzheimers Dement Pathological angiogenesis was associated with cerebrovascular lesion and neurodegeneration in Alzheimer's disease | ||
Pharmaceutics In Vivo Imaging of Acute Hindlimb Ischaemia in Rat Model: A Pre-Clinical PET Study | ||
Mol Cell Proteomics Profiling the Surfaceome Identifies Therapeutic Targets for Cells with Hyperactive mTORC1 Signaling. |
