Validation Data Gallery
|Positive WB detected in
|Positive IHC detected in
|human tonsillitis tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human kidney tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:3000-1:8000
|IHC : 1:5000-1:20000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66211-1-Ig targets CD13 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Mouse / IgG2a
|CD13 fusion protein Ag5976
|alanyl (membrane) aminopeptidase
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M-1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)
|Product Specific Protocols
|WB protocol for CD13 antibody 66211-1-Ig
|IHC protocol for CD13 antibody 66211-1-Ig
|IF protocol for CD13 antibody 66211-1-Ig
|FC protocol for CD13 antibody 66211-1-Ig
|Click here to view our Standard Protocols
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