CoraLite®488-conjugated CD13 Monoclonal antibody

CD13 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2D8D11

Cat no : CL488-66211

Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN



Tested Applications

Positive IF detected inhuman kidney tissue
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66211 targets CD13 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD13 fusion protein Ag5976
Full Name alanyl (membrane) aminopeptidase
Calculated Molecular Weight 110 kDa
GenBank Accession NumberBC058928
Gene Symbol ANPEP
Gene ID (NCBI) 290
RRIDAB_2883285
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174) And this antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

Protocols

Product Specific Protocols
IF protocol for CL488 CD13 antibody CL488-66211Download protocol
FC protocol for CL488 CD13 antibody CL488-66211Download protocol
Standard Protocols
Click here to view our Standard Protocols