CL488-conjugated CD13 Antibody

CD13 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2D8D11

Cat no : CL488-66211

Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN



Tested Applications

Positive IF detected inmouse kidney tissue
For other applications, we recommend the unconjugated version of this antibody, 66211-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66211 targets CL488-conjugated CD13 in IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD13 fusion protein Ag5976
Full Name alanyl (membrane) aminopeptidase
Calculated molecular weight 110 kDa
Observed molecular weight
GenBank accession number BC058928
Gene symbol ANPEP
Gene ID (NCBI) 290
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174) And this antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated CD13 antibody CL488-66211Download protocol
Standard Protocols
Click here to view our Standard Protocols