Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 55018-1-AP

Synonyms

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35



Tested Applications

Positive WB detected inC2C12 cell, HeLa cells, NIH/3T3 cells, HepG2 cells, C6 cells, K-562 cells
Positive IHC detected inhuman oesophagus tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55018-1-AP targets Annexin A1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name annexin A1
Calculated molecular weight 40 kDa
Observed molecular weight 39 kDa
GenBank accession numberNM_000700
Gene symbol ANXA1
Gene ID (NCBI) 301
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes. The antibody is specific to ANXA1.

Protocols

Product Specific Protocols
WB protocol for Annexin A1 antibody 55018-1-APDownload protocol
IHC protocol for Annexin A1 antibody 55018-1-APDownload protocol
FC protocol for Annexin A1 antibody 55018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Expert Rev Mol Diagn

Co-overexpression of Hsp90-β and annexin A1 with a significantly positive correlation contributes to the diagnosis of lung cancer.

Authors - Rong Biaoxue
humanIHC

Am J Transl Res

Elevated serum annexin A1 as potential diagnostic marker for lung cancer: a retrospective case-control study.

Authors - Biaoxue Rong
humanWB, IHC

J Exp Clin Cancer Res

Upregulation of Hsp90-beta and annexin A1 correlates with poor survival and lymphatic metastasis in lung cancer patients.

Authors - Biaoxue Rong R
mouseWB,IF

Mediators Inflamm

Interaction between ANXA1 and GATA-3 in Immunosuppression of CD4(+) T Cells.

Authors - Peng Huang
monkeyWB

J Proteomics

iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus.

Authors - Xiaozhen Guo