Annexin A1 Polyclonal antibody

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• Annexin A1 Polyclonal antibody (55018-1-AP)

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• KD/KO Validated

Annexin A1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55018-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

ANXA1, Annexin 1, Annexin Ac2-26, Annexin I, Annexin-1

• 

  WB analysis of C2C12 using 55018-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of C6 using 55018-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 55018-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 55018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 55018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 55018-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 55018-1-AP (Annexin A1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human oesophagus using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human oesophagus using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C2C12 cell, HeLa cells, NIH/3T3 cells, HepG2 cells, C6 cells, K-562 cells
• Positive IHC detected in: human colon tissue, human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55018-1-AP targets Annexin A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: annexin A1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: NM_000700
• Gene Symbol: Annexin A1
• Gene ID (NCBI): 301
• RRID: AB_10860266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04083
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes. The antibody is specific to ANXA1.

Publications

What published studies show

Annexin A1 antibody (Cat# 55018-1-AP) has 13 citations published in journals including Nature Communications, Journal of Experimental & Clinical Cancer Research, FASEB Journal, Journal of Medicinal Chemistry, Cellular and Molecular Biology Letters, Mediators of Inflammation, Journal of Cell Science, Neurotoxicity Research, Food Science & Nutrition, Expert Review of Molecular Diagnostics, Journal of Proteomics, and American Journal of Translational Research. Research fields span lung cancer, breast cancer, renal cell carcinoma, epithelial morphogenesis, immunology, blood-brain barrier biology, vascular disease, neurotoxicity, autophagy, and virology.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis; Authors - Maria Fankhaenel ; KD Validated
human	WB	J Exp Clin Cancer Res; YTHDC1 is downregulated by the YY1/HDAC2 complex and controls the sensitivity of ccRCC to sunitinib by targeting the ANXA1-MAPK pathway; Authors - Wei Li
human	WB,IHC	J Exp Clin Cancer Res; Upregulation of Hsp90-beta and annexin A1 correlates with poor survival and lymphatic metastasis in lung cancer patients.; Authors - Biaoxue Rong R
human,canine	WB	Cell Mol Biol Lett; AGR2-mediated unconventional secretion of 14-3-3ε and α-actinin-4, responsive to ER stress and autophagy, drives chemotaxis in canine mammary tumor cells; Authors - Stephen Hsien-Chi Yuan
human	WB	J Med Chem; Discovery of a Novel 1,4-Benzodiazepine Derivative as a Highly Selective ANXA3 Degrader for the Treatment of Triple-Negative Breast Cancer; Authors - Kunlin Liu
rat	IHC	Food Sci Nutr; Jiawei Danxuan Koukang and Its Component Kaempferol Alleviate Oral Submucous Fibrosis by Restoring Epithelial-Fibroblast Homeostasis and Suppressing Neutrophil Recruitment via the ANXA1/FPR2 Axis.; Authors - Yao Xiao