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Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3H1B11

Cat no : 60051-1-Ig

Synonyms

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I



Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, 4T1 cells, NIH/3T3 cells, HSC-T6 cells, HepG2 cells, HEK-293 cells, HeLa cells, A549 cells, A431 cells,
Positive IP detected inHeLa cells
Positive IF detected inT-47D cells, Hela cells, MCF-7 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

IPSee 1 publications below

Product Information

60051-1-Ig targets Annexin A2 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Annexin A2 fusion protein Ag1777
Full Name annexin A2
Calculated molecular weight 339 aa, 39 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC016774
Gene symbol ANXA2
Gene ID (NCBI) 302
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Protocols

Product Specific Protocols
WB protocol for Annexin A2 antibody 60051-1-IgDownload protocol
IHC protocol for Annexin A2 antibody 60051-1-IgDownload protocol
IF protocol for Annexin A2 antibody 60051-1-IgDownload protocol
IP protocol for Annexin A2 antibody 60051-1-IgDownload protocol
FC protocol for Annexin A2 antibody 60051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

EBioMedicine

Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.

Authors - Xiao Li

Infect Immun

The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation.

Authors - Xiaowei Wang