Tested Applications
| Positive WB detected in | K-562 cells, HEK-293 cells, THP-1 cells, A431 cells, A549 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue, human skin cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | T-47D cells, HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60051-1-Ig targets Annexin A2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1777 Product name: Recombinant human Annexin A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC016774 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD Predict reactive species |
| Full Name | annexin A2 |
| Calculated Molecular Weight | 339 aa, 39 kDa |
| Observed Molecular Weight | 35-38 kDa |
| GenBank Accession Number | BC016774 |
| Gene Symbol | Annexin A2 |
| Gene ID (NCBI) | 302 |
| ENSEMBL Gene ID | ENSG00000182718 |
| RRID | AB_2057309 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07355 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
| IHC protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
| IP protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
| WB protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Annexin A2 antibody (Cat# 60051-1-Ig) has accumulated 16 citations published in high-impact journals including Cell, Protein Cell, EBioMedicine, Apoptosis, British Journal of Cancer, Cell Signaling, iScience, Journal of Virology, Matrix Biology, and Infection and Immunity. Associated research fields span oncology (gastric, lung, melanoma, and prostate cancers), infectious disease, atherosclerosis, neurology, and fundamental cell biology processes such as cytokinesis, mitophagy, and endoplasmic reticulum homeostasis.
| Species | Application | Title |
|---|---|---|
Cell Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice | ||
Protein Cell NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody | ||
EBioMedicine Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder. | ||
Apoptosis Neural stem cell-derived exosomal PA2G4 induces ANXA2 degradation to promote mitophagy and alleviate neuronal oxidative stress in cerebral ischemia/reperfusion. | ||
Br J Cancer Quiescent cancer cells induced by high-density cultivation reveals cholesterol-mediated survival and lung metastatic traits | ||
Cell Mol Life Sci Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation |
